Microscopes

UA Cancer Center microscopes
Douglas Cromey, MS, TACMASR

Common core microscopes

Inverted microscope with color camera

Contact:  Doug Cromey for training
Location:  UACC 3908
Information:

· Zeiss Axiovert 200 with 1.5 optivar
· Axiocam MRC color camera (14 fps, 1388 x 1040 pixels)
· X-Cite metal halide lamp
· Available microscope objectives include: 5xPH/0.12, 10xPH/0.25, 20xPH/0.30, 40xPH/0.50, 100x/1.40 oil
· Computer uses Zeiss Axiovision software.

Imaging modes: Brightfield (transmitted light), Phase contrast, Fluorescence (DAPI, FITC/GFP, Rhodamine)

Shared Service Microscopes (fee-for-service)

iScan Coreo - Microscope Slide Scanner

Contact: TACMASR, 626-7319
Location:  UACC 0917
Information: Automated microscope slide scanner capable of scanning multiple slides in color, each scan stitches together multiple images using either: 5x, 10x, 20x, or 40x objective lenses, included software can be used for pathology scoring, or images can be exported in a file format suitable for image analysis using the Definiens software*.
Imaging modes: Brightfield (transmitted light)
Fee:  has not been determined yet

Confocal Microscope

Contact: TACMASR, Doug Cromey, 626-2824, cromey@arizona.edu
Training: required, contact Mr. Cromey for more information
Location:  UACC 0960
Information:

· Leica SP5-II spectral confocal microscope
· Regular & high-speed scanning
· Three detectors
· Laser lines of 405, 458, 477, 488, 514, 543, and 633nm
· Available microscope objectives include: 5x/0.15, 10x/0.4, 20x/0.7, 20x/07 multi-immersion, 40x/1.25 oil, 63x/1.40 oil. 
· The confocal includes an environmental chamber suitable for live cell imaging. 
· See: Confocal for additional information.

Imaging modes: Fluorescence (dyes similar to DAPI, FITC/GFP, Rhodamine, CY5), transmitted light (greyscale only)
Fee:  $32/hr
 

Outside the UACC building, but otherwise nearby core facilities

Inverted microscope with color & high-sensitivity greyscale camera

Contact:  Doug Cromey for training
Location:  LSN 429B
Information:

· Inverted Leica DMI6000 fully motorized microscope
· Leica DFC450 color (5Mpix) and Hamamatsu Flash 4.0 (4Mpix) 16bit greyscale camera (greater sensitivity to low light situations than a color camera)
· Available microscope objectives include: 2.5x/0.07, 5x/0.13, 10x/0.30, 20x/0.60, 40x/0.75 dry, 40x/1.00 oil, 100x/1.40 oil
· Software (Leica LAS-AF 3.x and Hamamatsu HCImage 4.x) – allows for ease of use, with the ability to capture and combine images of different types.  The software can be used to control the microscope which can stitch together multiple images into one large field of view.
· See: Microscopy Alliance Leica

Imaging modes:Brightfield (transmitted light), Phase contrast, DIC, Polarized light, Fluorescence (DAPI, FITC/GFP, Rhodamine, CY5)
Fee:  none now,possibly a fee of $4-5/hr beginning July 2014
 

Other campus core facility microscopes

(Light microscopes, Image analysis, Electron microscopes, Atomic Force microscopes, etc.)

· See: Microscopy Alliance

Technical questions about microscopes or related techniques?  Mr. Cromey has over 30 years of experience with light and electron microscopy, as well as image analysis and how to properly work with scientific digital images.  Contact him at (520) 626-2824 or dcromey@email.arizona.edu