Dr. Aspinwall is a professor in the Department Chemistry and Biochemistry and the Department of Biomedical Engineering at UA, a member of UA's BIO5 Institute for Collaborative Bioresearch, UA Cancer Center and UA Sarver Heart Center, and a participant in the Biological Chemistry Program and Training Grant. His research group synergystically focuses on the application of analytical chemistry to better understand biological systems and the subsequent application of biological knowledge and materials to develop new analytical technologies. The majority of their research efforts are focused on the development of biomimetic and biofunctionalized sensor strategies that allow detection of key signaling components, while also proving useful for other key bioanalyses including drug discovery and clinical diagnostics. Their long term goals are to develop a molecular level understanding of the chemical and physical processes involved in cellular signaling. Specifically, they are active in the areas of biofunctionalized nanomaterials, biochemical sensor design and chemical separations.
Dr. Aspinwall's group is one of only a few in the world that has the capabilities to regularly synthesize polymerizable phospholipid membranes from natural and synthetic materials and subsequently functionalize these membranes with functional ion channels, including ligand gated and chimeric ion channels, to make novel measurements. Coupled with their other sensors and separations geometries, they are at the forefront of integrating polymeric lipid biomaterials into real-world analysis platforms.
Dr. Aspinwall has worked extensively with pancreatic b-cells, b-cell lines and islets in my 20+ years in active research and actively collaborate with multiple diabetes research groups in the US and abroad. He has a demonstrated record of successful and productive research projects, as PI and co-investigator in the field of bioanalytical chemistry, cell biology and biomedical engineering.
- Zhou Y.; Bright L.K.; Shi W.; Aspinwall C.A.; Baker, L.A.”Ion channel probes for scanning ion conductance microscopy.” Langmuir, 30: 15351-15355, 2014 PMID: 25425190; PMCID: PMC4278694.
- Baker, C.A.; Bright, L.K.; Aspinwall, C.A. “Photolithographic fabrication of microapertures with well-defined, three-dimensional geometries for suspended lipid membrane studies.” Anal. Chem. 85:9078-9086, 2013. PMID: 23987300
- Aspinwall, C.A.; Qian, W.; Roper, M.G.; Kulkarni, R.N.; Kahn, C.R.; Kennedy, R.T. “Roles of insulin receptor substrate-1, phosphatidylinositol 3-kinase, and release of intracellular Ca2+ stores in insulin-stimulated insulin secretion in b-cells.” J. Biol. Chem. 275: 22331-22338, 2000. PMCID: PMCID not required.
- Qian, W.J.; Aspinwall, C.A.; Kennedy, R.T. “Temporally and spatially resolved measurement of Zn2+ efflux from pancreatic b-cells by confocal fluorescence microscopy.” Anal. Chem. 72: 711-717, 2000. PMCID: PMCID not required
- Bränström, R.; Leibiger, I.B.; Leibiger, B.; Aspinwall, C.A.; Corkey, B.E.; Berggren, P-O.; Larsson, O. “Single Residue (K332A) Substitution in Kir6.2 Abolishes the Stimulatory Effect of Long-Chain Acyl-CoA Ester: Indications for a Long-Chain Acyl-CoA Ester Binding Motif” Diabetologia, 50: 1670-1677, 2007. PMCID: PMCID not required.
- Aspinwall, C.A.; Huang, L.; Lakey, J.R.T.; Kennedy, R.T. “Comparison of amperometric methods for detection of exocytosis from single pancreatic beta-cells of different species.” Anal. Chem. 71: 5551-5556, 1999. PMCID: PMCID not required.
- Rauf, F.; Huang, Y.; Muhandiramlage, T.P.; Aspinwall, C.A. “Analysis of protein kinase A activity in insulin-secreting cells using a cell-penetrating protein substrate and capillary electrophoresis.” Anal. Bioanal. Chem. 397: 3359-67, 2010. PMCID: PMC 3064950.
- Aspinwall, C.A.; Lakey, J.R.T.; Kennedy, R.T. “Insulin-stimulated insulin secretion in single pancreatic b-cells.” J. Biol. Chem. 274: 6360-6365, 1999. PMCID: not required.
- Heitz, B.A.; Xu, J.; Hall, H.K., Jr.; Aspinwall, C.A.; Saavedra, S.S. “Enhanced long-term stability for single ion channel recordings using suspended poly(lipid) bilayers" J. Am. Chem. Soc., 131: 6662-6663, 2009. PMCID: PMC2682430
- Heitz, B.A.; Jones I.W.; Hall, H.K. Jr.; Aspinwall, C.A.; Saavedra, S.S. “Fractional polymerization of a suspended planar bilayer creates a fluid, highly stable membrane for ion channel recordings.” J. Am. Chem. Soc. 132: 7086-7093, 2010. PMCID: PMC2882248.
- Bright, L.K.; Baker, C.A.; Agasid, M.T.; Ma, L.; Aspinwall, C.A. “Decreased aperture surface energy enhances electrical, mechanical, and temporal stability of suspended lipid membranes.” ACS Appl. Mater. Interfaces, 5: 11918-11926, 2013 PMID: 24187929; PMCID: PMC3909927.
- Heitz, B.A.; Xu, J.; Jones, I.W.; Keogh, J.P.; Comi, T.J.; Hall, H.K.; Aspinwall, C.A.; Saavedra, S.S. “Polymerized Planar Suspended Lipid Bilayers for Single Ion Channel Recordings: Comparison of Several Dienoyl Lipids.” Langmuir, 27:1882-1890, 2011. PMCID: PMC3043114.
- Gallagher, E.S.; Adem, S.; Baker, C.A.; Ratnayaka, S.; Jones, I.W.; Hall, Jr., H.K., Saavedra, S.S.; Aspinwall, C.A. “Highly Stabilized Phospholipid Bilayers as Stationary Phases for Capillary Liquid Chromatography” J. Chrom. A. 1385: 28-34, 2015; PMID: 25670414
- Gallagher, E.S.; Mansfield, E.M.; Aspinwall, C.A. “Stabilized Phospholipid Membranes in Chromatography: Towards Membrane Protein-Functionalized Stationary Phases” Anal. Bioanal. Chem., 406: 2223-2229, 2014. PMCID: PMC3969766
- Gallagher, E.S.; Adem, S.M.; Bright, L.K.; Calderon, I.A.C.; Mansfield, E.; Aspinwall, C.A. “Hybrid Phospholipid Bilayer Coatings for Separations of Cationic Proteins in Capillary Zone Electrophoresis” Electrophoresis, 35: 1099-1105, 2014 PMID: 24459085; PMCID: PMC4041483.
- Mansfield, E.; Ross, E.E.; Keogh, J.P.; D’Ambruoso, G.D.; Huang, Y.; Aspinwall, C.A. “Fabrication and characterization of spatially-defined, multiple component chemically-functionalized domains in enclosed silica channels using cross-linked phospholipid membranes” Langmuir, 23: 11326-11333, 2007, PMID: 17892310; PMCID: PMC2654225.
- Roberts, D.L.; Ma, Y.; Bowles, S.E.; Janczak, C.M.; Pyun, J.; Saavedra, S.S.; Aspinwall, C.A. “Polymer-stabilized phospholipid vesicles with a controllable, pH-dependent disassembly mechanism” Langmuir, 25: 1908-1910, 2009. PMCID: PMC2654230.
- Muhandiramlage, T.P.; Cheng, Z.L.; Roberts, D.L.; Keogh, J.P. ; Hall Jr.,H.K.; Aspinwall, C.A., "Determination of pore sizes and relative porosity in porous nanoshell architectures using dextran retention with single monomer resolution and proton permeation", Anal. Chem., 84: 9754-9761, 2012. PMID: 23083108, PMCID: PMC3502667.
- Cheng, Z.; D’Ambruoso, G.D.; Aspinwall, C.A. “Stabilized Porous Phospholipid Nanoshells” Langmuir 22: 9507-9511, 2006. PMCID: PMCID not required.
- Li, Z; ;Muhandiramlage, T.P.; Keogh, J.P.; Hall, Jr., H.K.; Aspinwall, C.A. “Aptamer-functionalized porous phospholipid nanoshells for direct measurement of Hg2+ in urine” Anal. Bioanal. Chem., 407: 953-960, 2015 .PMCID: PMC4305462.
2014-Present Associate and Full Professor, Biomedical Engineering, University of Arizona, Tucson, AZ
2009 Visiting Professor, The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Karolinska University Hospital, Stockholm Sweden
2008-Present Affiliate, Sarver Heart Center, University of Arizona
2006-Present Member, BIO5 Institute, University of Arizona, Tucson, AZ
2002-Present Assistant, Associate and Full Professor, Department of Chemistry and Biochemistry, University of Arizona, Tucson, AZ
2000-2001 Alexander Hollaender Distinguished Postdoctoral Research Fellow, Ames Laboratory, U.S. Dept. of Energy, Iowa State University (Advisor: Prof. Edward S. Yeung), Ames, Iowa
1999-2000 Postdoctoral Research Fellow, Department of Molecular Medicine, Karolinska Institute (Advisor: Prof. Olof Larsson), Stockholm, Sweden
1994-1999 Graduate Research Assistant, Department of Chemistry, University of Florida (Advisor: Prof. Robert T. Kennedy), Gainesville, Florida
1993 Intern chemist, Research and Development Department, Mydrin, Inc., Calhoun, Georgia;
1992 NSF Research Experience for Undergraduates, Department of Chemistry, University of Alabama, Tuscaloosa, Alabama
2015 IUPAC US National Committee Young Observer
2015 Organizing Committee, GTCBio Bioanalytical Sensors Conference
2011-Present Member, International Advisory Board, Analytical and Bioanalytical Chemistry, Chromatography and Separations Chemistry
2011 ACS GREET (Global Research Experiences, Exchanges, and Training) Scholar
2010-2012 Member, A-Page Features Panel, Analytical Chemistry
2009 ACS Division of Analytical Chemistry Award for Young Investigators in Separation Science
2008 Keynote Speaker, International Chemistry Conference, Taipei
2005-2011 NSF CAREER Award
2003 ACS Leadership Development Award
2002-2005 3M Nontenured Faculty Award
2000-2002 NIH NRSA Fellow; inactive
2000-2001 DOE Alexander Hollaender Distinguished Postdoctoral Fellow
1999-2000 Swedish Institute Cultural Exchange Scholar
1997-1998 ACS Division of Analytical Chemistry Full Year Fellow