George Tsaprailis, PhD

Director, Proteomics Shared Resource
Staff Scientist
Assistant Research Scientist, BIO5 Institute
Phone Number: 
(520) 626-5461
(520) 626-6944

UACC Information

Professional Bio: 

Dr. Tsaprailis was recruited in 2001 as Director of the Proteomics Shared Resource and, in 2006, was also appointed Director of the Arizona Proteomics Consortium. He holds a PhD in biochemistry with significant work experience in biological MS (especially LC-MS) and with significant training and expertise in mechanisms of gas-phase peptide fragmentation in a mass spectrometer using a variety of activation methods. As a result of his post doctoral work, new insights into peptide fragmentation mechanisms were obtained and new rules for peptide / protein sequencing based on tandem MS have been incorporated into database searching algorithms. His knowledge in fundamental and applied biological MS gives him the necessary capabilities to evaluate and interpret MS data for users. In addition, Dr. Tsaprailis’ training in biochemistry has proven beneficial, as he is familiar with biochemical techniques used by UACC investigators and how these techniques complement the proteomics approach to protein analysis. He has demonstrated leadership in obtaining extramural funding to support the proteomics efforts of the UACC, SWEHSC, and other units on campus.  He has also successfully acquired new proteomics instrumentation for the Proteomics Shared Resource through competitive instrumentation grants (4000 QTRAP – funded in 2006, QTOF Premier – funded in 2008, and LTQ Orbitrap Velos – funded in 2010).

Dr. Tsaprailis teaches Proteomics every fall in the Department of Pharmacology and Toxicology and also holds regural mini-proteomics workshopd for UACC Members. He is also a an Assistant Research Scientist at the BIO5 Institute.

Classically trained in chemistry and biochemistry with an emphasis in enzymology, Dr. Tsaprailis gained LC-MS/MS experience in the mid-1990s as a graduate student working on identifying sites of radical formation on cytochrome c peroxidase and later on by setting up and managing a biological mass spectrometry core laboratory at Concordia University. Post-doctoral training through a fellowship from FCAR with world-renowned mass spectrometrist Professor Vicki H. Wysocki at the University of Arizona allowed for a greater understanding of peptide fragmentation in the gas phase and of mass spectrometry in general, and resulted in five peer-reviewed publications. This invaluable experience ushered his position in proteomics at the University of Arizona, which at that time, was beginning to invest heavily in this area. Dr. Tsaprailis has worked in this capacity since 2000 and has interacted with many graduate students, post-doctoral fellows and faculty, helping with their mass spectrometry and proteomics needs in almost all areas of science research. Dr. Tsaprailis' interest in state-of-the-art proteomics applications and technology has fostered working relationships with leaders in this area in the academic and private sectors, and he has brought many new proteomics capabilities and hardware to the University of Arizona campus since 2000.

Dr. Tsaprailis continues to serve as the Director of the Proteomics Facility Core of the SWEHSC, the Director of the Univeristy of  Arizona Cancer Center Proteomics Shared Resource and also as Director of the Arizona Proteomics Consortium. He oversees the daily operations of the various proteomics facilities, am an operator of LCQ, LTQ, LTQ Orbitrap Velos, and QTOF LC-MS/MS system performing proteomics analyses, assist with proteomics data analysis and interpretation or proteomics core users, conduct proteomics workshops regularly.

Dr. Tsaprailis assists with grant writing, serves as PI on various proteomics core center grants, instruct a graduate class in Proteomics in the Department of Pharmacology and Toxicology, serves as guest lecturer for undergraduate biochemistry, and is currently on the committee of four graduate students, and supervises four employees.

Research Information

Research Program: 
Cancer Biology
Selected Publications: 

Professional Information

Other Experience and Professional Memberships/Affiliations: 
2014              Special Emphasis Panel Reviewer, ZRG1 BCMB-D30, Shared Mass Spectral Instruments, NIH/CSR
2013              Manuscript Reviewer, Mol. Cell. Prot.
2012              Special Emphasis Panel Reviewer, ZRG1 BCMB-D30, Shared Mass Spectral Instruments, NIH/CSR
2012              Manuscript Reviewer, J. Proteome Res.
2011              Manuscript Reviewer, Int. J. Mass Spectrom.
2010              Special Emphasis Panel Reviewer, ZCA1 SRLB-R (J1) R, Clinical Proteomics Technologies for Cancer Initiative Research, NIH/NCI
2010              Manuscript Reviewer, Chemico Biol. Interact.
2009              Special Emphasis Panel Reviewer, ZRG1 BCMB-D30 I, Shared Mass Spectral Instruments, NIH/NCRR
2009              PO1 Special Emphasis Panel Reviewer, ZGM1 PPBC-O (AN), NIGMS
2009              Challenge Grants Panel 5 Reviewer, ZRG1 BCMB-P (58)R, NIH
2008              PO1 Special Emphasis Panel Reviewer, Molecular Oncology, NCI
2006 -           Member, BIO5 Institute, University of Arizona
2006              PO1 Reviewer, Molecular Oncology 2, NCI
2003, 2011   Manuscript Reviewer, JASMS
2003 -           Associate Member, Arizona Cancer Center
2000 - 2004 Peptide Synthesis Research Group Member, Association of Biomolecular Resources Facilities
2000 -           Member, Southwest Environmental Health Sciences Center
1997 -           Member, American Society for Mass Spectrometry

Academic Information

Post Doctoral: 
University of Arizona, Tucson, AZ
Concordia University, Montreal, PQ Canada
Undergraduate School: 
McGill University, Montreal, PQ Canada