Associate Research Professor
July 2005 – present
University of Arizona, Bio5, Tucson, AZ, Associate Research Professor, Director of Ligand Discovery Lab; Advanced Research Institute for Biomedical Imaging (member)
High throughput synthesis and screening of diverse chemical libraries.
Design of ligands for disease diagnosis and therapy.
Design and synthesis of labeled biomarkers and pharmaceuticals.
May 2002 – June 2005
University of Arizona, Bio5, Tucson, AZ, Assistant Research Professor (Organic Chemist)
Scientific advisers: Prof. Robert J. Gillies and Prof. Victor J. Hruby
The design and solid-phase synthesis of multimeric ligands for binding to the combination of epitopes specific to human cancer cells.
December 1998 – September 2001
SIDDCO, Tucson, AZ, Scientific Fellow 5 (Synthetic Organic Chemist/Principal Scientist)
The design and synthesis of combinatorial libraries in a format suitable for high throughput screening as potential drugs. The development and optimization of reactions and scaffolds on solid-phase supports (the protocols to be used in the production of combinatorial libraries).
November 1995 – September 1998
Novo Nordisk A/S, Copengagen, Denmark, Research Chemist
The design and synthesis small combinatorial libraries – arrays of organic compounds, and multiple synthesis in automatic devices. The development of reactions on solid-phase support, multi-step organic syntheses, purifications and analyses of products.
October 1992 - October 1995
University of Minnesota, Department of Chemistry, Minneapolis, USA; Postdoctoral Fellow
Scientific adviser: Prof. George Barany
The solid-phase peptide synthesis involving the synthesis of defensins, orthogonal protection schemes, acid-labile handles and protecting groups. The combinatorial chemistry for synthesis of non-peptide libraries.
Currently, I am leading the Ligand Discovery Lab (LDL) at Bio5, University of Arizona. The current research projects deal with the development of ligands, inhibitors, and diagnostic probes in collaboration with the AZCC, UNMCC, TGen, Department of Radiology and Department of Biochemistry.
- Solid-phase synthesis (small organic compounds, biopolymers)
- High throughput synthesis (96-well plates)
- One-bead-one-compound (OBOC) library synthesis
- Rational design of small molecule ligands, multimeric ligands, peptidomimetics
- Lanthanide-based time-resolved fluorometry in vivo binding assay
- In vivo cell-adhesion-to-bead assay for OBOC library
- HT synthesis of labeled probes (fluorescent, DOTA-Eu)
- Logistics and QC
Vagner, J., Barany, G., Lam, K.S., Krchnak, V., Sepetov, N.F., Ostrem, J.A., Strop, P., and Lebl, M., Enzyme-mediated spatial segregation on individual polymeric support beads: application to generation and screening of encoded combinatorial libraries. Proceedings of the National Acadademy of Science of the U.S.A., 93, 8194-8199 (1996).
The LDL proram relies on an existing information flow from TGen, and collaborations with other groups:
Victor J Hruby (UA,pancreatic cancer, melanoma),
Gene Mash (UA,pancreatic cancer, melanoma),
Bob Gillies (Moffitt, pancreatic/breast cancer and melanoma),
Bill Monfort (UA, angiogenesis),
Ron Lynch (UA, diabetes),
Sean Limesand (UA, diabetes),
Anne Cress (AZCC, prostate cancer),
Art Gmitro (Dept. of Radiology, esophagus cancer),
Scott Boitano (Bio5, astma),
Marilyn Halonen (UA, astma) ,
Michael Daines (UA, astma),
Jeff Walker (UA, cardiovascular),
Marty Pagel (UA, MRI).
The American Chemical Society
The American Peptide Society
The Society of Combinatorial Sciences
2006 GI SPORE Career Development Award
1993-1995 Selectide (Sanofi-Aventis) Postdoctoral Fellowship