The focus of Dr. Sharma's research is to determine the mechanisms by which mutations in splicing factors lead to a disease phenotype in hematopoietic malignancies, with a focus on Myelodysplastic syndromes (MDSs). They use cord blood derived hematopoietic stem and progenitor cells (HSPCs) to study the effect of MDS-linked splicing factor mutations on the myeloid differentiation pathways. These studies involve high-throughput RNA-sequence analysis for determining splicing profile changes in HSPCs due to expression of MDS-mutations. They are also carrying out proteomic analyses to determine changes in proteins interactions due to mutations.
As a member of the University of Arizona Cancer Center (UACC), Dr. Sharma's work has been supported by the UACC shared resources, particularly the Genomics Shared Resource and the Proteomics Shared Resource. She is a member of the Cancer Biology Program and attend the program meetings. Presentations and interactions with other members at these meetings have been good for obtaining feedback on research projects and for initiating collaborations.