The focus of Dr. Sharma's research is to determine the mechanisms by which mutations in splicing factors lead to a disease phenotype in hematopoietic malignancies, with a focus on Myelodysplastic syndromes (MDSs). They use cord blood derived hematopoietic stem and progenitor cells (HSPCs) to study the effect of MDS-linked splicing factor mutations on the myeloid differentiation pathways. These studies involve high-throughput RNA-sequence analysis for determining splicing profile changes in HSPCs due to expression of MDS-mutations. They are also carrying out proteomic analyses to determine changes in proteins interactions due to mutations.
As a member of the University of Arizona Cancer Center (UACC), Dr. Sharma's work has been supported by the UACC shared resources, particularly the Genomics Shared Resource and the Proteomics Shared Resource. She is a member of the Cancer Biology Program and attend the program meetings. Presentations and interactions with other members at these meetings have been good for obtaining feedback on research projects and for initiating collaborations.
- Wongpalee S and Sharma S (2013) The pre-mRNA splicing reaction, Klemens Hertel (ed.) Methods in Molecular Biology series, Humana press.
- Keppetipola N*, Sharma S*, Li Q, and Black DL (2012) Neuronal regulation of pre-mRNA splicing by polypyrimidine tract binding proteins, PTBP1 and PTBP2, Critical Reviews in Biochemistry and Molecular Biology 47, 360-78. (*First co-authors). PMCID: 22655688.
- Sharma S, Maris C, Allain F H-T, and Black DL (2011) U1 snRNA directly interacts with polypyrimidine tract binding protein during splicing repression, Mol.Cell 41, 579-88. PMCID:21362553.
- Tang ZZ, Sharma S, Zheng S, Chawla G, Nikolic J, and Black DL (2011) Regulation of the mutually exclusive exons 8a and 8 in the CaV1.2 calcium channel transcript by polypyrimidine tract binding protein, J. Biol Chem. 286, 10007-16. PMCID:3060452
- Babic I, Sharma S, and Black DL (2009) A role for polypyrimidine tract binding protein in the establishment of focal adhesions, Mol. Cell. Biol. 29, 5564-77. PMCID :19667078.
- Sharma S, Kohlstaedt L, Damianov A, Rio DC, and Black DL (2008) Polypyrimidine tract binding protein controls transition from exon definition to an intron defined spliceosome, Nat. Struct. Mol. Biol. 15, 183-191. PMCID: 2546704.
- Sharma S (2008) Isolation of polypyrimidine tract binding protein using RNA affinity chromatography, Ren-Jang Lin (ed.) RNA-Protein Interaction Protocols, second edition, Humana press. PMCID: 18982280.
- Sharma S and Black DL (2006) Maps, Codes, and Sequence Elements: Can We Predict the Protein Output from an Alternatively Spliced Locus? Neuron 52, 574-576.
- Sharma S, Falick AM and Black DL (2005) Polypyrimidine Tract Binding Protein Blocks the 5 Splice Site Dependent Assembly of U2AF and the Prespliceosomal E Complex, Mol. Cell 19, 485-496. PMCID: 1635971.