Developed / Implemented Assays

Below is a list of assays developed or implemented in the Analytical Chemistry.

Analyte

Sample Type

Instrument

 4-HPR (N-(4-hydroxyphenyl)-retinamide)

Plasma; Tissue

HPLC-FLD 

8-Hydroxy-2'-deoxyguanosine

Urine; DNA 

HPLC-MS/MS 

8-Isoprostaglandin F2 alpha 

Urine; Plasma

HPLC-MS/MS

Acetylcholine

Mouse cortex

HPLC-MS/MS

Alpha-tocopheryloxyacetic acid

Plasma

HPLC-MS/MS

AMP423

Formulation Preparation

HPLC-MS 

Antalarmin 

Plasma 

HPLC-MS/MS 

Arginine/Ornithine

Intestine; Colon Tissue

HPLC-FLD 

Bile acids

Fecal Water; Plasma; Intestinal fluid

HPLC-MS 

Bile acids (parent bile acids and taurine and glycine conjugates)

Gastric fluid

HPLC-MS/MS 

Buspirone 

Plasma 

HPLC-MS/MS 

Caffeine/Caffeine metabolites 

Plasma 

HPLC-UV;HPLC-MS/MS

Carbendazim 

Plasma 

HPLC-MS/MS 

Carotenoids

Plasma; Skin 

HPLC-FLD 

Celecoxib 

Plasma 

HPLC-UV 

Chlorophyll 

Colon Tissue 

HPLC-FLD

Cortisol/6-Hydroxycortisol 

Urine

HPLC-UV 

Cortisol/Cortisone 

Saliva

HPLC-MS/MS 

Curcumin

Plasma; Urine; Skin 

HPLC-UV; HPLC-MS/MS

Dexmedetomidine

Urine

HPLC-MS/MS 

Dextromethorphan (DM)/DM metabolites

Urine 

HPLC-FLD 

DFMO (Alpha-difluoromethylornithine)

Plasma; Skin 

HPLC-FLD

DIM (diindolylmethane)

Urine

HPLC-MS/MS

Diphenidol

Cell culture media

HPLC-MS/MS

DITPA (3,5-diiodothyropropionic acid) 

Plasma 

HPLC-MS/MS

Dopamine

Mouse Cortex

HPLC-MS/MS

DPC0046

Plasma

HPLC-MS/MS

Epinephrine

Mouse cortex

HPLC-MS/MS

Estradiol/estrone

Plasma; urine

HPLC-MS/MS

Fatty acids

Plasma

HPLC-MS/MS

GABA (gamma-aminobutyric acid)

Mouse Cortex; Urine

HPLC-MS/MS 

gamma-Glutamylcysteine 

Cell extract 

HPLC-FLD 

Gemcitabine Triphosphate

Blood Lymphocytes; Cell Extract

HPLC-UV

Gemcitabine 

Plasma; Tissue; Cell Extract 

HPLC-UV; HPLC-MS/MS

Glutamate

Mouse Cortex

HPLC-MS/MS 

Glyoxal, Methyl glyoxal

Cell extract

GC-MS

HETEs (hydroperoxyeicosatetraenoic acid)

Lung Tissue 

HPLC-MS/MS

Imexon

Plasma 

HPLC-UV 

Isothiocyanate-glutathione conjugates

Cultured cells 

HPLC-MS/MS 

Limonene 

Plasma; breast tissue, adipose tissue 

HPLC-MS/MS; GC-MS 

Losartan/Losartan Metabolites

Urine; Plasma

HPLC-UV;HPLC-MS/MS

Malondialdehyde

Cell extract

GC-MS

Melphalan/Melphalan analogues

Dosing Preparation 

HPLC-MS/MS

Metformin

Plasma; Cell culture media; Tissue

HPLC-MS/MS 

Midazolam/ Midazolam Metabolites

Plasma

HPLC-MS/MS

Nitotinic acid/Nicotinamide

Plasma 

HPLC-MS/MS

Nitrite

Cell culture media

HPLC-FLD

Norepinephrine

Mouse cortex

HPLC-MS/MS

NPA-093

Plasma

HPLC-UV

Omeprazole/Metabolites

Plasma

HPLC-MS/MS

Pemetrexed (Alimta®)

Plasma; IP fluid

HPLC-MS/MS

Pentosidine

Plasma

HPLC-FLD

Phenethylamine

Urine

HPLC-MS/MS

PHT-427

Plasma; Tissue

HPLC-UV; HPLC-MS/MS

PMIP (therapeutic peptide)

Plasma

HPLC-MS/MS 

Polyamines

Plasma; Tissue

HPLC-FLD 

Psorospermin methyl ether

Plasma

HPLC-UV 

PX-12 

Plasma 

HPLC-UV; HPLC-MS/MS

Quercetin

Plasma; skin

HPLC-MS/MS

Radicicol

Plasma

HPLC-MS/MS

Rapamycin

Whole blood

HPLC-MS/MS

Resveratrol

Plasma; Tissue

HPLC-MS/MS 

Retinols

Plasma; Skin 

HPLC-FLD

Selenium

Plasma, prostate tissue 

AA 

Sulindac/Sulindac metabolites

Plasma; Breast Fluid; Skin 

HPLC-MS/MS 

Targretin

Plasma

HPLC-FLD 

Taurine

Urine

HPLC-MS/MS

Tea Catechins

Plasma; Urine 

HPLC-EC 

Testosterone

Plasma

HPLC-MS/MS 

Thyroxine/Triiodothyroxine

Plasma 

HPLC-MS/MS 

Tocopherol/Tocopherol succinate

Plasma; Tissue 

HPLC-FLD

UA8967

Plasma

HPLC-MS/MS

Withaferin analogues

Plasma

HPLC-MS/MS 

Wortmannin analogues

Plasma; Bile

HPLC-MS/MS

PDF of table


In addition to the listed methods, the Analytical Chemistry Shared Resource can develop analytical procedures for determination of novel chemicals in biological samples and adapt additional published analytical procedures.