The Flow Cytometry Shared Resource (FCSR) at The University of Arizona Cancer Center supports the research needs of all Cancer Center members by providing state-of-the-art instrumentation for data acquisition, analysis, and cell sorting, and the technical expertise to interpret results, and develop methods. We offer information about new techniques, and applications of flow cytometry through workshops and seminars, and provide training to interested facility users who wish to run their own samples. Individual consultation services are available to discuss the specifics of each project.
Under the Direction of Amanda Baker, PhD, and Caroline Garcia, Associate Vice President for Research, the FCSR is operated and administered as a partnership between The University of Arizona Cancer Center and Arizona Research Laboratories, Division of Biotechnology.
Flow cytometry is a powerful tool that measures the functional and structural characteristics of heterogeneous mixtures of cells and particles in suspension based on their ability to scatter light. The cell sorting function separates these cells physically into their different classes. Researchers have the capability to analyze and sort cells by differences in physiology, metabolism, morphology and other characteristics. The ability to distinguish different cell types is limited only by the ability to attach specific fluorescent markers to the cells.
Four instruments are available for use:
The FACScanto II (BD Biosciences, San Jose, CA) is an analyzer capable of acquiring and analyzing data from six fluorescence parameters and has two lasers: 488nm and 640nm.
The FACSAria III (BD Biosciences, San Jose, CA) is a cell sorter equipped with 5 lasers: 488nm, 633nm, 561nm, 405nm and 355nm, and offers 17 fluorescence detectors. The sorting function of this instrument enables the researcher to physically separate the individual cells and particles from a mixed population. Viable cells can be recovered for further study or culture. The addition of a Baker BioProtect III biosafety cabinet, in which the Aria is housed, allows for sorting of certain live human tissue and BSL2 agents.
The LSRII (BD Biosciences, San Jose, CA) is an analyzer capable of acquiring and analyzing data from 16 fluorescence parameters and has five lasers: 355nm, 405nm, 488nm, 532 nm and 640nm.
Paula Campbell, Manager
John Fitch, Core Operator
Amanda Baker, PhD, Director
Caroline Garcia, Associate Vice President, Research